Mucosal and humoral immune responses following infectious bronchitis virus in naïve guinea fowl


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Project Details

Type of project: KFU Raed Track

Start date: 27/09/2021

End date: 27/09/2022


Abstract

  • Infectious bronchitis virus (IBV) is a highly contagious pathogen that causes respiratory, renal and reproductive diseases in chickens. The virus primarily replicates in the epithelial tissues of the respiratory tract, such as turbinate and trachea. Little information is available on immune responses in other birds like guinea fowls. For this reason, we will examine the local, humeral immune responses as well as the viral IBV detection in the multiple tissues/ organs of guinea fowls experimentally infected with IBV. 6 month age-old guinea fowls will be divided into two groups and infected via the oculonasal route as follows: (i) group one will be inoculated with live attenuated IBV (M41) (0.1 ml of 106 EID50/chick) and (ii) group two will be mock-inoculated with 0.1 ml of virus-free allantoic fluid. Lachrymal fluid will be collected for anti-IBV IgA. In addition, serum will be also collected from whole blood for systemic immunity by IgY testing. Turbinate, trachea, cecal tonsils and kidney tissues will be collected. RNA will be extracted from above tissues for virus detection using real Time and Conventional RT-PCR.


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Last updated on 2021-14-10 at 08:04